Entry 24 of 279 from Query 20   * Names only * * Complete entries * CABRI_Cell_Lines_View CABRI_Std_View

Entry Information           Entry from: ICLC     Entry Options           Link to related information: Save entry: View:

CABRI:ICLC Accession_number ICLC ATL99010 Cell_line_name B16-F10 Brief_description Species: mouse, C57BL/6; Tumor: melanoma Description Species: mouse, C57BL/6; Tumor: melanoma Depositor Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy Bibliographic_reference Cancer Res 1975;35:218-224 [PMID: 1109790 ] Morphology epithelial-like, grown as monolayer Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 Viruses Search for viruses was not performed Properties The cells produce melanin Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard - Species_validation Validated by isoenzymes: confirmed as mouse with NP, PepB Tumorigenicity tumorigenic and metastatic in syngeneic animals Freezing_medium Culture medium + 50% FBS + 10% DMSO Sterility mycoplasma negative, HOECHST and PCR Further_bibliography Nature 1973;242:148-149

SRS Release 7.1.3    Copyright © 1997-2003 LION bioscience AG. All Rights Reserved. Terms of Use Feedback